哪种从头装配算法最适合我的数据? 希拉里·米勒(Hilary Miller)2020年9月22日7:46 Geneious中提供了许多汇编程序。某些汇编器未与Geneious捆绑在一起,但可以通过Tools-> Plugins作为可选插件安装。最好使用的汇编程序可能取决于您的数据。下面是一些汇编程序的优缺点的简要概述。Geneious Advantages: - Produces large contigs - Produces contigs containing reads - Can produce list of unused reads - Can produce circular contigs 缺点: - 慢(不适用于超过 100 Mbp 的基因组) - 高内存使用率 蝌蚪(Geneious R9 起)好处: - Extremely fast - Very low mis-assembly rate Disadvantages: - Produces only consensus sequences - May produce shorter contigs - May not work as well on gappy error models (e.g. 454, IonTorrent, PacBio) - Does not produce scaffolds SPAdes(Geneious 10.1 以上) 优点: - Produces long and accurate contigs - Works with many data types (Note:Oxford Nanopore,PacBio和Sanger读取只能用于具有较高质量的短读取数据的混合组件中。 - Supports RNA and metagenome data Disadvantages: - Produces only consensus sequences - Doesn't work with low coverage - Not designed for large genomes Flye(Prime 2020.1 以后的插件) 优点: - 快速地- 专为 PacBio 和 Nanopore 数据而设计- 适用于宏基因组数据 缺点: - 不支持短读(低于 1000 bp) - 仅产生一致序列 天鹅绒(插件)好处: - 快速地- 被广泛使用的- 在更大的基因组上比 Geneious 组装器更有效 缺点: - Produces only consensus sequences - May not work as well on gappy error models (e.g. 454, IonTorrent, PacBio)MIRA (plugin)Advantages: - Works very well on bacterial genomes - Produces large contigs - Produces contigs containing reads 缺点: -在大型基因组上不可行 以上内容来自互联网,仅供中文用户参考,仍未解决?马上联系中文技术支持→→→提交难题/咨询/报错 购买正版Geneious Prime,享受随时技术支援,免费提供中文操作教程、含正规发票! 立即购买正版Geneious Prime软件(淘宝店铺)