如何反转补体引物？

Primers are always 5’ to 3’ so in Geneious if you reverse complement a primer, the sequence viewer will show the other strand and the primer direction arrow will switch from left to right to right to left.在文本视图中，您应该看到引物实际上并没有改变，仍然是原始序列。If you really want to switch the primer to the other strand it needs to be run through ’Characteristics for Selection’ again since the annotated primer now doesn’t correspond to the sequence.It is worth deleting the current primer annotations and then running the ’Characteristics for Selection’ tool, which will create a new primer annotation running from left to right that corresponds to the sequence as it now exists.