对primer3 计算的API 访问

这是一个您可以复制的方法，它调用我们的primer3 插件：

/**
 * 调用与“Characteristics for Selection...”相同的操作，使用primer3 计算Tm
 * @param 序列
* @return 解链温度或 -1 如果序列为空
* @throws DocumentOperationException 例如，如果序列对于primer3 来说太长（超过36bp）
 */
私有静态双计算熔化温度（CharSequence 序列）抛出 DocumentOperationException {
 if (sequence == null || sequence.length() == 0) return -1;
 if (sequence.length() > 36) throw new DocumentOperationException("Sequence too long for prime3");

 SequenceAnnotationGenerator generator = PluginUtilities.getSequenceAnnotationGenerator("com.biomatters.plugins.primerDesign.CharacteristicsAnnotationGenerator");
 if (generator == null) throw new DocumentOperationException("未启用初级设计插件。");

 AnnotatedPluginDocument apdDoc = DocumentUtilities.createAnnotatedPluginDocument(new DefaultNucleotideSequence("temp", sequence));
 SequenceAnnotationGenerator.SelectionRange selectionRange = new SequenceAnnotationGenerator.SelectionRange(0, 0, 0, sequence.length() - 1);
列表<List<SequenceAnnotation> > 特征注释；
选项 annealingCharacteristicOptions = generator.getOptions(new AnnotatedPluginDocument[] {apdDoc}, selectionRange);
 featureAnnotations = generator.generateAnnotations(selectionRange, ProgressListener.EMPTY, annealingCharacteristicOptions, apdDoc);
 String tm =characteristicAnnotations.get(0).get(0).getQualifierValue("Tm");
 //其他特征如发夹可以在这里检索
return Double.parseDouble(tm.split("-")[0]);
 }